microglycodb_gene_00052
| Gene Number | BLLJ_1841 |
|---|---|
| UniProt ID | A0A401ETL2 |
| NCBI Protein ID | FAA01256.1 |
| Protein Name | |
| Enzyme Name | exo-β-1,6-galactanase (renamed exo-β-1,6-galactobiohydrolase) |
| CAZy Family | GH30_5 |
| Reviewed | true |
| Organism | Bifidobacterium longum |
sequence
functions
publications
reaction
Data not found
Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851). Specifically releases the non-reducing terminal beta-1,6-galactobiose (beta-1,6-Gal2) from both dearabinosylated larch AG and polymeric beta-1,6-galactan chains by an exo-mode of action (PubMed:30564851). Shows lower activity with larch AG, and very weak activity with dearabinosylated gum arabic, gum arabic and potato galactan (PubMed:30564851). Can probably release beta-1,6-Gal2 from the internal side chains of type II AG (PubMed:30564851).
kcat is 261 sec(-1) with beta-1,6-Gal3 as substrate.
Optimum pH is 4.5 with beta-1,6-Gal3 as substrate.
Optimum temperature is 40 degrees Celsius with beta-1,6-Gal3 as substrate.
10 20 30 40 50
MRVLSKSLAA MVAAATLVGG GAFAVAGTAY AADNDAITVT PNPWYANSFD
60 70 80 90 100
GWGTSLAWFA NATGSLGEES AITTNLGDDA SKAKAVEYGK QLREQFYQSI
110 120 130 140 150
FGDEGLDLNM ARYNVGGGNA SDVAYGYPFM RQGAAVPGTW KDDATGSGTY
160 170 180 190 200
GNGVTTKQAD KDKLAAAFDP TDDNQYDFSK SAAQDWWIER GATGDNPDIT
210 220 230 240 250
DVEAFANSAP WFLTNSGYAT GGRNSGSNNL ANPEKFAQYM AKNVEHLESL
260 270 280 290 300
GANVDTVEPF NESETSYWGT PGDMASKYTD ESDDNTKLIN NYWDKYYSDK
310 320 330 340 350
DRSVTPYSNA LKKPQEGMHV SNAQQQQTIT ALAEALKDND DTIIAATDAT
360 370 380 390 400
NSADFVKSYN QYPQAIKDLI GQYNVHAYSD SNQMQSRDIA QADGKKLSMS
410 420 430 440 450
EVDGSWQSGS YNPYGFDNAL GMMSKISSNV TRLQSKDFTF WQVVEDLYNM
460 470 480 490 500
QMGSNVNPAG ENTNWGTVLI DFDCTVAGMD GKLYSERRVN NNGGTTDGLE
510 520 530 540 550
PCTVIANAKY NGVKAITHFI HAGDKVIANN DEDNNMTATS DDGKTQTVIH
560 570 580 590 600
RNSGTSDQTF VIDLSKYGEI ADNAYGELYL TTETSAEDKN AGVDSATPEV
610 620 630 640 650
FAKTSNVKQA EGSVMIDKAA KTATVTVPAR SIASIQLTGV TGYAKDAAVE
660 670 680 690 700
TGDTYQLVGK QSGKAVADTT SGDSALSLAN VASDAENAKK QTWTFTQIEQ
710 720 730 740 750
PTDSERPDLK AYVITNAEGK VLVSKDGTNA LSNETVGAAK SDPAAKWILN
760 770 780 790 800
TSDGSTYQLL NAATKTNLDV DNSGTTVGTK VGLWQSPSGT SPSANQTWTL
810 820 830 840 850
RNVTPTSQKT VNVQTAVNEK AVLPVEVTLY YTWGEGKATV ANWDTSKVDV
860 870 880 890 900
AKEGAYEATA TATDVYGNEF NVTATVYVGA LTVSDPVSAT VLAGTSASEA
910 920 930 940 950
KAALEAAPVY LHVKASPAFE GDAAKVTWNF DGLDTKLADA KAGDNIAVTG
960 970 980 990 1000
TYQLDDATTI ALKGAIYVTA ATPENVADTA SNLTVTNQQT EYSKGDQWKK
1010 1020 1030 1040 1050
LTDGDTSAEA WVTWNSAGDY SASPTATIDF GSECELSSVT ITYGDKAPAS
1060 1070 1080 1090 1100
AKAEYTTDGE TWMQFGSDVK PAAGQTVTFK ADKGTVNATK MRIVNTVNND
1110 1120 1130 1140 1150
YMNATEIQAF VTPVQGAAKN IAAASGTNFS VNFQEGASAS KAIDGDTTSK
1160 1170 1180 1190 1200
GWSTWASTAS TVDPVATFTF DEAQTITEVK TFFYYDGRAS WPKSQTLEYQ
1210 1220 1230 1240 1250
DEAGEWHGVG TKDGWKIQAG DAGSGSDGIT AADTPTVDFV LGTPVKAKAI
1260 1270 1280 1290 1300
RLTNTLQDTK VYINVAEIQV FAQDSTVLTP QPASDATLGD LRLDGETVEG
1310 1320 1330 1340 1350
FDPSKTDYTV DLPVDAEANP VLQAFATDNA AAVKVTGDAV ENGKLGGKAA
1360 1370 1380 1390 1400
ITVTSADESE TKTYTVTFNA FTLASLKVIG PTKTEYAIGD KLDTAGLKVT
1410 1420 1430 1440 1450
AVYQSGDKTK EVPVALDDPQ LAIGSFDSTT AGKKAITVSY RGVTATFNVT
1460 1470 1480 1490 1500
VKANAVAPGP EEQKPGNTNK PGATGNGNKN TVANTGSSVA AIAGAVALLA
1510 1520
AAAGALFMLR KRA