MicroGlycoDB

microglycodb_gene_00053

Gene Number	BLLJ_1854
UniProt ID	A0A3R0A696
NCBI Protein ID	FAA01257.1
Protein Name
Enzyme Name	α-L-arabinofuranosidase
CAZy Family	GH43_22
Reviewed	true
Organism	Bifidobacterium longum

sequence

functions

publications

reaction

Data not found

Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851). Releases arabinofuranose (Araf) from alpha-1,3-Araf-substituted beta-1,6-galactooligosaccharides (PubMed:30564851). Can use radish root AGP, larch AG and arabinan. Shows weaker activity with gum arabic and arabinoxylan (PubMed:30564851).

kcat is 220 sec(-1) with larch arabinogalactan as substrate.

Optimum pH is 5.5 with larch arabinogalactan as substrate.

Optimum temperature is 50 degrees Celsius with larch arabinogalactan as substrate.

        10         20         30         40         50

MKHWKKMAAS LIAISTMVAV VPTTYAMESE DSQPQTTDTA TVQTTKAAEP

        60         70         80         90        100

TLLASWDFTG KNGTTNSAIA DSTGKYNLTL KDGAKIEQYG DRSNNEALSL

       110        120        130        140        150

RGDGQYAQID DQLFKDAGDS FTLEFASKTR HDDSGKFFSF IVGKDGSNDA

       160        170        180        190        200

NTTDQANANK YLMFYNSKTA IKGVISNNNW GNEQGSKVTV SGNDNSWADY

       210        220        230        240        250

KIVVDGTNLA VFRNNALIIF KANTGIKMSD LGATTAYIGK SFYSVDEYWN

       260        270        280        290        300

GAMDDIKVYR GADLTMPTAV AISGTGVVNN KLTLIEKDST KLTATVTPDD

       310        320        330        340        350

AVSKNVTWSS SDESVAKVAA DGTVTGVKAG TATITATTEL GGVKAELPVT

       360        370        380        390        400

VEPMNAQNAA AADLDAAIAA LKVPAAENLP LVAKGTKNGS AITWKSSDEK

       410        420        430        440        450

LITSTNEKYE NRTTGADDPY RGAGIINRPA YGDGDSKPVT LTATASYNGG

       460        470        480        490        500

EKVTKTIEVT VKEKTRIAPD TGYAAVTFES DSNGGEKAWV ASTEKNDFFT

       510        520        530        540        550

FKTRNNGQAV LTNDADTGGL RDMFVLRSHE GDKYYLIATD LKVSSMGWSQ

       560        570        580        590        600

NQVNGSRKVE VYESTDMMNW TRTNGDGNGG ITINTPNAGM TWAPEAYWDD

       610        620        630        640        650

DLNAYVVFFS SRMFTDDTRT TPVKNDKTGN SSYAQVRYAI TRDFVNFTEP

       660        670        680        690        700

QMWQDTGYSR IDSTVRKIGG YYYRFTKNEQ GGAAGDYITT GKSIFLERSK

       710        720        730        740        750

VLTAPTTEAS PGQDPNTGWQ LLEQALLPFE GPETIKLNKD DELNTKDDDG

       760        770        780        790        800

YILLSDNFAY RAFMTTGAEL SKTTWDNPMT KRYPDFNNEK KPVKAEPGAQ

       810        820        830        840        850

GYITQGANGG LPDKVRHGAF VNVPESVLKV TKSWTAANPT HIEAVDSTTK

       860        870        880        890        900

AVYNAGTREL TATVTSADKG TLAGSVKFSA GDWSKTVKLD AEGKATVTLP

       910        920        930        940        950

ASVSGTVAVA YDGYTDGLVN PSDTTVDGIE QGKVDLAELN KQIAAAEALK

       960        970        980        990       1000

ESDYTADSWA KLAAALKTAK AALAAENQGE VDTAAADLKT AIEALQKAPT

      1010       1020       1030       1040       1050

NPGEGDGDKG DGNKPTTPTT GDKTNVNKPG SALSNTGTAV LGLGGAVVAL

      1060       1070

AIAGISLTLW RKRRA

MKHWKKMAASLIAISTMVAVVPTTYAMESEDSQPQTTDTATVQTTKAAEPTLLASWDFTGKNGTTNSAIADSTGKYNLTLKDGAKIEQYGDRSNNEALSLRGDGQYAQIDDQLFKDAGDSFTLEFASKTRHDDSGKFFSFIVGKDGSNDANTTDQANANKYLMFYNSKTAIKGVISNNNWGNEQGSKVTVSGNDNSWADYKIVVDGTNLAVFRNNALIIFKANTGIKMSDLGATTAYIGKSFYSVDEYWNGAMDDIKVYRGADLTMPTAVAISGTGVVNNKLTLIEKDSTKLTATVTPDDAVSKNVTWSSSDESVAKVAADGTVTGVKAGTATITATTELGGVKAELPVTVEPMNAQNAAAADLDAAIAALKVPAAENLPLVAKGTKNGSAITWKSSDEKLITSTNEKYENRTTGADDPYRGAGIINRPAYGDGDSKPVTLTATASYNGGEKVTKTIEVTVKEKTRIAPDTGYAAVTFESDSNGGEKAWVASTEKNDFFTFKTRNNGQAVLTNDADTGGLRDMFVLRSHEGDKYYLIATDLKVSSMGWSQNQVNGSRKVEVYESTDMMNWTRTNGDGNGGITINTPNAGMTWAPEAYWDDDLNAYVVFFSSRMFTDDTRTTPVKNDKTGNSSYAQVRYAITRDFVNFTEPQMWQDTGYSRIDSTVRKIGGYYYRFTKNEQGGAAGDYITTGKSIFLERSKVLTAPTTEASPGQDPNTGWQLLEQALLPFEGPETIKLNKDDELNTKDDDGYILLSDNFAYRAFMTTGAELSKTTWDNPMTKRYPDFNNEKKPVKAEPGAQGYITQGANGGLPDKVRHGAFVNVPESVLKVTKSWTAANPTHIEAVDSTTKAVYNAGTRELTATVTSADKGTLAGSVKFSAGDWSKTVKLDAEGKATVTLPASVSGTVAVAYDGYTDGLVNPSDTTVDGIEQGKVDLAELNKQIAAAEALKESDYTADSWAKLAAALKTAKAALAAENQGEVDTAAADLKTAIEALQKAPTNPGEGDGDKGDGNKPTTPTTGDKTNVNKPGSALSNTGTAVLGLGGAVVALAIAGISLTLWRKRRA

1. Bifidobacteria can protect from enteropathogenic infection through production of acetate.

PubMed: 21270894

doi:10.1038/nature09646

2. Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum.

PubMed: 30564851