microglycodb_gene_00058

Gene Number BLLJ_0211
UniProt ID E8MGH8
NCBI Protein ID BAK79118.1    
Protein Name
Enzyme Name β-L-arabinofuranosidase
CAZy Family GH127
Reviewed true
Organism Bifidobacterium longum
sequence
functions
publications
reaction
Data not found

 Beta-L-arabinofuranosidase that removes the beta-L-arabinofuranose residue from the non-reducing end of various substrates, including beta-L-arabinofuranosyl-hydroxyproline (Ara-Hyp), Ara-beta-1,2-Ara-beta-Hyp (Ara(2)-Hyp), Ara-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara(3)-Hyp), and beta-L-arabinofuranosyl-(1->2)-1-O-methyl-beta-L-arabinofuranose. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.

 kcat is 2.0 sec(-1) with beta-Ara2. kcat is 0.013 sec(-1) with Ara-Hyp. kcat is 6.3 sec(-1) with Ara(2)-Hyp.

Data not found
Data not found

        10         20         30         40         50

MNVTITSPFW KRRRDQIVES VIPYQWGVMN DEIDTTVPDD PAGNQLADSK 

        60         70         80         90        100

SHAVANLKVA AGELDDEFHG MVFQDSDVYK WLEEAAYALA YHPDPELKAL 

       110        120        130        140        150

CDRTVDLIAR AQQSDGYLDT PYQIKSGVWA DRPRFSLIQQ SHEMYVMGHY 

       160        170        180        190        200

IEAAVAYHQV TGNEQALEVA KKMADCLDAN FGPEEGKIHG ADGHPEIELA 

       210        220        230        240        250

LAKLYEETGE KRYLTLSQYL IDVRGQDPQF YAKQLKAMNG DNIFHDLGFY 

       260        270        280        290        300

KPTYFQAAEP VRDQQTADGH AVRVGYLCTG VAHVGRLLGD QGLIDTAKRF 

       310        320        330        340        350

WKNIVTRRMY VTGAIGSTHV GESFTYDYDL PNDTMYGETC ASVAMSMFAQ 

       360        370        380        390        400

QMLDLEPKGE YADVLEKELF NGSIAGISLD GKQYYYVNAL ETTPDGLDNP 

       410        420        430        440        450

DRHHVLSHRV DWFGCACCPA NIARLIASVD RYIYTERDGG KTVLSHQFIA 

       460        470        480        490        500

NTAEFASGLT VEQRSNFPWD GHVEYTVSLP ASATDSSVRF GLRIPGWSRG 

       510        520        530        540        550

SYTLTVNGKP AVGSLEDGFV YLVVNAGDTL EIALELDMSV KFVRANSRVR 

       560        570        580        590        600

SDAGQVAVMR GPLVYCAEQV DNPGDLWNYR LADGVTGADA AVAFQADLLG 

       610        620        630        640        650

GVDTVDLPAV REHADEDDAP LYVDADEPRA GEPATLRLVP YYSWANREIG 

       660

EMRVFQRR




1. Bifidobacteria can protect from enteropathogenic infection through production of acetate.

PubMed: 21270894

doi:10.1038/nature09646


2. Characterization of a novel beta-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member.

PubMed: 21914802

doi:10.1074/jbc.m111.248690


3. Characterization of a novel beta-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 protein family member.

PubMed: 24385433

doi:10.1074/jbc.m113.528711


4. Crystal structure of glycoside hydrolase family 127 beta-l-arabinofuranosidase from Bifidobacterium longum.

PubMed: 24680821

doi:10.1016/j.bbrc.2014.03.096


5. Crystallization and preliminary X-ray diffraction analysis of a novel beta-L-arabinofuranosidase (HypBA1) from Bifidobacterium longum.

PubMed: 24817727

doi:10.1107/s2053230x14001812


6. Structure and catalytic mechanism of a glycoside hydrolase family-127 beta-L-arabinofuranosidase (HypBA1).

PubMed:

doi:10.4172/2155-9821.1000171