MicroGlycoDB

microglycodb_gene_00145

Gene Number	Cj1126c
Gene Name	pglB
UniProt ID	Q0P9C8
NOTES - phase variation etc	Oligosaccharide transferase to N-glycosylate proteins and release FOS
Sugar/modification added	OTase
Reviewed	true
Biosynthesis	N-glycan biosynthesis
Organism	Campylobacter jejuni

functions

sequence

publications

reaction

Data not found

Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (GalNAc(2)GlcGalNAc(3)Bac(NAc)(2) in eubacteria, where Bac(NAc)(2) is di-N-acetyl bacillosamine) from the lipid carrier undecaprenol-pyrophosphate to an asparagine residue within an Asp/Glu-Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation.

        10         20         30         40         50

MLKKEYLKNP YLVLFAMIVL AYVFSVFCRF YWVWWASEFN EYFFNNQLMI

        60         70         80         90        100

ISNDGYAFAE GARDMIAGFH QPNDLSYYGS SLSTLTYWLY KITPFSFESI

       110        120        130        140        150

ILYMSTFLSS LVVIPIILLA NEYKRPLMGF VAALLASVAN SYYNRTMSGY

       160        170        180        190        200

YDTDMLVIVL PMFILFFMVR MILKKDFFSL IALPLFIGIY LWWYPSSYTL

       210        220        230        240        250

NVALIGLFLI YTLIFHRKEK IFYIAVILSS LTLSNIAWFY QSAIIVILFA

       260        270        280        290        300

LFALEQKRLN FMIIGILGSA TLIFLILSGG VDPILYQLKF YIFRSDESAN

       310        320        330        340        350

LTQGFMYFNV NQTIQEVENV DFSEFMRRIS GSEIVFLFSL FGFVWLLRKH

       360        370        380        390        400

KSMIMALPIL VLGFLALKGG LRFTIYSVPV MALGFGFLLS EFKAILVKKY

       410        420        430        440        450

SQLTSNVCIV FATILTLAPV FIHIYNYKAP TVFSQNEASL LNQLKNIANR

       460        470        480        490        500

EDYVVTWWDY GYPVRYYSDV KTLVDGGKHL GKDNFFPSFS LSKDEQAAAN

       510        520        530        540        550

MARLSVEYTE KSFYAPQNDI LKSDILQAMM KDYNQSNVDL FLASLSKPDF

       560        570        580        590        600

KIDTPKTRDI YLYMPARMSL IFSTVASFSF INLDTGVLDK PFTFSTAYPL

       610        620        630        640        650

DVKNGEIYLS NGVVLSDDFR SFKIGDNVVS VNSIVEINSI KQGEYKITPI

       660        670        680        690        700

DDKAQFYIFY LKDSAIPYAQ FILMDKTMFN SAYVQMFFLG NYDKNLFDLV

       710        720

INSRDAKVFK LKI

1. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.

PubMed: 10688204

doi:10.1038/35001088

2. Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni.

PubMed: 16356848

doi:10.1016/j.chembiol.2005.10.004

3. Overexpression and topology of bacterial oligosaccharyltransferase PglB.

PubMed: 20331969