MicroGlycoDB

microglycodb_gene_00148

Gene Number	Cj1129c
Gene Name	pglH
UniProt ID	Q0P9C5
NOTES - phase variation etc	Alpha-1,4-N-acetyl-galactosaminyltransferase, GlcNAc-polymerase
Sugar/modification added	3 x alpha 1-4 GalNAc
Reviewed	true
Biosynthesis	N-glycan biosynthesis
Organism	Campylobacter jejuni

functions

sequence

publications

reaction

Data not found

Processive glycosyltransferase that is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation. Catalyzes the transfer of exactly three alpha-(1->4)-N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenyl (PubMed:16186480, PubMed:19159314, PubMed:29386647). Cannot accept UDP-GlcNAc as substrate (PubMed:29386647).

        10         20         30         40         50

MMKISFIIAT LNSGGAERAL VTLANALCKE HEVSIIKFHA GESFYKLENE

        60         70         80         90        100

VKVTSLEQFR FDTLYHKIAS RFKKFFALRK ALKESKSDVF ISFLDTTNIA

       110        120        130        140        150

CIAAKIGLKT PLIISEHSNE AYLKPKIWRF LRRVSYPFCD ALSVLGSSDK

       160        170        180        190        200

VYYERFVKRV KLLLNPCHFS DEISFDSSFE KENLVLFIGR LDHNKNPVMF

       210        220        230        240        250

LKAIAHLDKN LQENYKFVIA GDGQLRQELE YKVKSLGIKV DFLGRVENVK

       260        270        280        290        300

ALYEKAKVLC LCSFVEGLPT VLIESLYFEV CRISSSYYNG AKDLIKDNHD

       310        320        330        340        350

GLLVGCDDEI ALAKKLELVL NDENFRKELV NNAKQRCKDF EISHIKEEWL

KLIAEVKNA

1. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.

PubMed: 10688204

doi:10.1038/35001088

2. In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation.

PubMed: 16186480

doi:10.1073/pnas.0507311102

3. Campylobacter jejuni PglH is a single active site processive polymerase that utilizes product inhibition to limit sequential glycosyl transfer reactions.

PubMed: 19159314

doi:10.1021/bi802284d

4. Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.

PubMed: 29386647