MicroGlycoDB

microglycodb_gene_00149

Gene Number	Cj1130c
Gene Name	pglK
UniProt ID	Q0P9C4
NOTES - phase variation etc	Flips LLO from cytoplasm into the periplasm
Sugar/modification added	Flippase
Reviewed	true
Biosynthesis	N-glycan biosynthesis
Organism	Campylobacter jejuni

functions

sequence

publications

reaction

Data not found

Mediates the ATP-dependent translocation of the undecaprenylpyrophosphate-linked heptasaccharide intermediate across the cell membrane; this is an essential step during the N-linked protein glycosylation pathway. Transport across the membrane is effected via ATP-driven conformation changes. Most likely, only the polar and charged part of the glycolipid enter the substrate-binding cavity, and the lipid tail remains exposed to the membrane lipids during the transmembrane flipping process.

        10         20         30         40         50

MLKKLFFILS KEDKNFLFFL LVFSVFISFI ETFAISLVMP FITLASDFSY

        60         70         80         90        100

FDRNKYLISL KEYLNIPVFE IIVYFGVGLI VFYVFRALLN AYYFHLLARF

       110        120        130        140        150

SKGRYHAIAY KVFSKFLNIN YEKFTQKNQS EILKSITGEV YNLSTMISSF

       160        170        180        190        200

LLLMSEIFVV LLLYALMLLI NYKITLFLSI FMVLNAFILV KILSPIIKKA

       210        220        230        240        250

GVRREEAMKN FFEILNTNLN NFKFIKLKTK EDGVLSLFKA QSEAFSKANI

       260        270        280        290        300

TNESVAAVPR IYLEGIGFCV LVFIVVFLVL KNESDISGIL STISIFVLAL

       310        320        330        340        350

YRLMPSANRI ITSYHDLLYY HSSLDIIYQN LRQEEENLGE EKLSFNQELK

       360        370        380        390        400

ICNLSFGYEG KKYLFKNLNL NIKKGEKIAF IGESGCGKST LVDLIIGLLK

       410        420        430        440        450

PKEGQILIDE QELNANNTKN YRQKIGYIPQ NIYLFNDSIA KNITFGDAVD

       460        470        480        490        500

EEKLNRVIKQ ANLEHFIKNL PQGVQTKVGD GGSNLSGGQK QRIAIARALY

       510        520        530        540        550

LEPEMLVLDE ATSALDTQSE AKIMDEIYKI SKDKTMIIIA HRLSTITQCD

       560        570

KVYRLEHGKL KEEK

1. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.

PubMed: 10688204

doi:10.1038/35001088

2. Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides.

PubMed: 16498400

doi:10.1038/sj.emboj.7601024

3. Biosynthesis of the N-linked glycan in Campylobacter jejuni and addition onto protein through block transfer.

PubMed: 16547029

doi:10.1128/jb.188.7.2427-2434.2006

4. Structure and mechanism of an active lipid-linked oligosaccharide flippase.

PubMed: 26266984

doi:10.1038/nature14953

5. The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116.

PubMed: 9720026