MicroGlycoDB

microglycodb_gene_00156

Gene Number	Cj1418c
Gene Name
UniProt ID	Q0P8J6
NOTES - phase variation etc	L-glutamine kinase
Sugar/modification added	Nucleotide-activated phosphoramidate biosynthesis
Reviewed	true
Biosynthesis	Capsular polysaccharide biosynthesis
Organism	Campylobacter jejuni

functions

sequence

publications

reaction

Data not found

Involved in the biosynthesis of the O-methyl phosphoramidate (MeOPN) group found on the capsular polysaccharide (CPS) of C.jejuni (PubMed:17675288). Catalyzes the ATP-dependent phosphorylation of the amide nitrogen of L-glutamine to form L-glutamine phosphate, the first committed step in MeOPN biosynthesis (PubMed:28650156). Does not phosphorylate L-glutamate or L-asparagine (PubMed:28650156).

        10         20         30         40         50

MAELKFKTKA QNLKNLQTKL KKAKVLPLVL TSLEELISNE DKVLQDIQTL

        60         70         80         90        100

KANRLIIRSS SLSEDSMKNS NAGAFLSLAN IKADSKDELL KALYEVANSM

       110        120        130        140        150

PSKSDEILVQ PMLENITLCG VGFSVDKDNF SPYFCLQYDE NGSNSSITDG

       160        170        180        190        200

SSKSAKTYYH YRNYLEFKDI RLQKIIELIK ELEVLYDCHF LDVEFAFAIQ

       210        220        230        240        250

DDKEELFCLQ VRPLVMHEKN NLFHSLPKEA LYRFYKRFES LKESRSRVLG

       260        270        280        290        300

DKAIFGVMPD WNPAEIIGLR PKRLAFSLYK EIITDNIWAY QRDNYGYRDL

       310        320        330        340        350

RSHPLIHSFL GIPYVDVRLS FNSFIPKKLD ENIAQKLVNF YLDKLNKNHE

       360        370        380        390        400

LHDKIEFNIV YSCYDFNSSK KLEELLNHGF NENEIKRLEF SLLELTNKII

       410        420        430        440        450

NPRSGFYLKD IQKAYKLKER YDGIINSNFS LIDKIYWLIE ECKRYGTLPF

       460        470        480        490        500

AGVARAAFVA MQLLNSLVEI DFITKEEKDD FLNSLNTVSK NLSKQTNHLN

       510        520        530        540        550

FHNKDQFLKD FGHLRAGTYN ILSPRYDEDF ELYFDADQKD SKVYLQDKAF

       560        570        580        590        600

VFSKEKTRAL NALLKEHGLE INACEFFDFL KQAIEGRELV KFEFTRLLSK

       610        620        630        640        650

AIVYIEELGK YYDIEKEDLA HLDIKSILNL YSSLYSINPK EQFIEEINRN

       660        670        680        690        700

KKEYELTQAI KLPSLLCNAD EIFSFYNHSI IPNFITQKSI TAFTAKENDK

       710        720        730        740        750

DLEGKIVLIY AADPGYDYLF TKNIAGLITC YGGANSHMAI RASELGMPAV

       760        770        780

IGVGEENFEK YLKAKKINIE CESEQIFCL

1. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.

PubMed: 10688204

doi:10.1038/35001088

2. Commonality and biosynthesis of the O-methyl phosphoramidate capsule modification in Campylobacter jejuni.

PubMed: 17675288

doi:10.1074/jbc.m704413200

3. Discovery of a glutamine kinase required for the biosynthesis of the O-methyl phosphoramidate modifications found in the capsular polysaccharides of Campylobacter jejuni.

PubMed: 28650156